Product Overview
HHb (Deoxyhemoglobin) represents the populous faction of bloodstream transport globulin proteins in its unliganded, deoxygenated state. Structurally configured as a non-covalently associated tetramer consisting of two α (141 amino acids) and two β (146 amino acids) subunits, this macromolecular matrix features an allosteric “Tense” (T) state characterized by a wide central water cavity and distinct low-affinity coordination chemistry toward gaseous ligands.
In analytical biochemistry and preclinical in vitro models, HHb serves as a baseline reference standard to investigate cooperative ligand binding kinetics, allosteric transition mechanisms to the “Relaxed” (R) state, and pH buffering capacities mediated by histidine residues. Its precise metalloprotein matrix acts as a specialized substrate for monitoring oxidation-reduction reactions, iron release kinetics, and protein-ligand interaction behaviors under variable environment models.
Technical Specifications
| Parameter | Specification |
|---|---|
| Chemical Profile | Human Hemoglobin (Deoxygenated Tetramer Matrix) |
| Subunit Architecture | Two α chains (141 aa) + Two β chains (146 aa) |
| Active Heme Centers | Four Protoporphyrin-IX Coordination Rings with Ferrous Iron (Fe²⁺) |
| Allosteric State | T-state (Tense Configuration / Unliganded Reference) |
| Purity Standard | Analytical Grade Reference Standard / Spectrophotometrically Verified |
| Physical Format | Sterile lyophilized matrix powder / Stabilized aqueous solution |
Laboratory Handling & Storage Guidelines
- Reconstitution: Reconstitute using sterile, thoroughly deoxygenated laboratory water or pH-targeted physiological buffers to prevent premature autoxidation. Avoid violent mechanical shaking to protect the tertiary and quaternary structural integrity of the protein tetramer.
- Long-Term Storage: Store the raw lyophilized reference compound desiccated at -20°C to -80°C. Protect meticulously from atmospheric oxygen, humidity variations, and temperature spikes to minimize spontaneous methemoglobin conversion.
- Solution Stability: Reconstituted working assays must be evaluated promptly or stored in anaerobic aliquots at ultra-low temperatures (-80°C) to mitigate structural denaturation and iron breakdown pathways.
⚠️ Regulatory Compliance & Mandatory Disclaimer
IMPORTANT NOTICE: This product is synthesized and distributed exclusively as a pure research standard for laboratory evaluation, academic investigation, and in vitro experimental models. IT IS STRICTLY NOT FOR HUMAN CONSUMPTION, DIAGNOSTIC, THERAPEUTIC, OR COSMETIC APPLICATIONS. All purchasing entities must represent certified academic or scientific research professionals.
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